Structure ofMycoplasma salivariumExamined by the Freeze Substitution Method
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چکیده
منابع مشابه
Structure of dictyosomes in Scenedesmus acuminatus observed by the freeze-substitution method.
The ultrastructure of dictyosomes in Scenedesmus acuminatus was studied by the freeze-substitution method. The cisternal membranes of dictyosomes clearly exhibited three-layered structures. Electron-dense particles were visible on or in the proximal cisternal membranes. The upper and lower membranes of the distal cisternae were in close contact with each other at the cisternal centre. The elect...
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Freeze-drying is one of the most used methods for preparing scaffolds and is very sensitive to the material and operational parameters such as nucleation temperature, thermal properties of the mold, cooling rate, set freezing point, and slurry height. In the present study, a Finite Element Method (FEM) based code was developed to investigate the effects of such parameters and to eventually ...
متن کاملMethods and Principles of Fixation by Freeze-Substitution
Freeze-substitution is based on rapid freezing of tissues followed by solution ("substitution") of ice at temperatures well below O degrees C. A 1 to 3 mm. specimen was thrown into 3:1 propane-isopentane cooled by liquid nitrogen to -175 degrees C. (with precautions). The frozen tissue was placed in substituting fluid at -70 degrees C. for 1 week to dissolve ice slowly without distorting tissue...
متن کاملEpoxy resin as fixative during freeze-substitution.
An alternative protocol for freeze-substitution is described. Araldite/Epon embedding medium (20% in acetone) is first used as a stabilizer (as e.g., OsO(4)) and then as embedding medium. The major components of the Araldite/Epon resin formulation react with proteins and lipids and provide for an excellent preservation and reasonable visualisation of the ultrastructure. The ultrastructural appe...
متن کاملThe Staining of Thin Sections of Mouse Pancreas Prepared by the Fernández-morán Helium Ii Freeze-substitution Method
Small pieces of mouse pancreas were rapidly frozen in helium II, substituted in methanol at -75 degrees C., and embedded in methacrylate by ultraviolet polymerization in the cold. The unstained cells show a structure similar to that after OsO(4) fixation, except that the RNP particles have little or no contrast and the mitochondria and Golgi zones appear as grey areas without internal structure...
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ژورنال
عنوان ژورنال: Microbiology and Immunology
سال: 1986
ISSN: 0385-5600
DOI: 10.1111/j.1348-0421.1986.tb03009.x